PISD Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84212

Novus Biologicals, part of Bio-Techne
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NBP1-84212
NBP1-84212-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PISD Antibodies »

Product Summary for PISD Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YKSVPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHSISPSD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PISD Antibody

Western Blot: PISD Antibody [NBP1-84212]

Western Blot: PISD Antibody [NBP1-84212]

Western Blot: PISD Antibody [NBP1-84212] - Analysis in human adipose tissue tissue.
Immunocytochemistry/ Immunofluorescence: PISD Antibody [NBP1-84212]

Immunocytochemistry/ Immunofluorescence: PISD Antibody [NBP1-84212]

Immunocytochemistry/Immunofluorescence: PISD Antibody [NBP1-84212] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & the Golgi apparatus.
Immunohistochemistry-Paraffin: PISD Antibody [NBP1-84212]

Immunohistochemistry-Paraffin: PISD Antibody [NBP1-84212]

Immunohistochemistry-Paraffin: PISD Antibody [NBP1-84212] - Staining of human placenta shows cytoplasmic positivity in trophoblasts.

Applications for PISD Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PISD

PISD, also referred to as Phosphatidylserine Decarboxylase proenzyme, is cleaved into two mature subunits called Phosphatidylserine decarboxylase alpha chain and Phosphatidylserine decarboxylase beta chain. The PISD proenzyme contains a LGST motif that is an autocatalytic cleavage site where it splits into the alpha chain and beta chain. PISD is part of the phosphatidylserine decarboxylase family and localizes to the mitochondrion. Current research on PISD is being conducted in relation to several diseases and disorders including carcinomas and malaria. Research has shown that PISD interacts with PMM2, SGPL1, DHX29, CRLS1 and NAPEPLD in pathways such as the FOXA1 transcription factor network, the metabolism of lipids and lipoproteins and glycerophospholipid metabolism.

Alternate Names

DJ858B16, dJ858B16.2, DKFZp566G2246, EC 4.1.1.65, phosphatidylserine decarboxylase, phosphatidylserine decarboxylase proenzyme, PSD, PSDC, PSSC

Gene Symbol

PISD

Additional PISD Products

Product Documents for PISD Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PISD Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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