PIST Antibody (2B1P9)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15280
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2B1P9
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PIST (Q9HD26). MSAGGPCPAAAGGGPGGASCSVGAPGGVSMFRWLEVLEKEFDKAFVDVDLLLGEIDPDQADITYEGRQKMTSLSSCFAQLCHKAQSVSQINHKLEAQLVD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PIST Antibody (2B1P9)
Western Blot: PIST Antibody (2B1P9) [NBP3-15280]
Western Blot: PIST Antibody (2B1P9) [NBP3-15280] - Western blot analysis of extracts of various cell lines, using PIST Rabbit mAb (NBP3-15280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunocytochemistry/ Immunofluorescence: PIST Antibody (2B1P9) [NBP3-15280]
Immunocytochemistry/Immunofluorescence: PIST Antibody (2B1P9) [NBP3-15280] - Immunofluorescence analysis of NIH-3T3 cells using PIST Rabbit mAb (NBP3-15280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: PIST Antibody (2B1P9) [NBP3-15280]
Immunohistochemistry-Paraffin: PIST Antibody (2B1P9) [NBP3-15280] - Immunohistochemistry of paraffin-embedded human thyroid cancer using PIST Rabbit mAb (NBP3-15280) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for PIST Antibody (2B1P9)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIST
Alternate Names
CALPDZ protein interacting specifically with TC10, CFTR-associated ligand, dJ94G16.2, FIGdJ94G16.2 PIST, Fused in glioblastoma, golgi-associated PDZ and coiled-coil motif containing, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC1, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, PISTGolgi associated PDZ and coiled-coil motif containing protein
Gene Symbol
GOPC
Additional PIST Products
Product Documents for PIST Antibody (2B1P9)
Product Specific Notices for PIST Antibody (2B1P9)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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