PIST Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1375
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-LDDLHTLYHKKSY corresponding to C-Terminus according to NP_065132.1, NP_001017408.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_065132.1; NP_001017408.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for PIST Antibody
![Western Blot: PIST Antibody [NB100-1375]](https://resources.bio-techne.com/images/products/PIST-Antibody-Western-Blot-NB100-1375-img0001.jpg "Western Blot: PIST Antibody [NB100-1375]")
Western Blot: PIST Antibody [NB100-1375]
Western Blot: PIST Antibody [NB100-1375] - Analysis of PIST/FIG/GOPC on Human Frontal Cortex lysate using this antibody at 0.05 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for PIST Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:128000
Western Blot
0.05 - 0.2 ug/ml
Application Notes
IF successful usage on HeLa cells. WB: Approx. 55 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 50.5 kDa band according to NP_065132.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PIST
Alternate Names
CALPDZ protein interacting specifically with TC10, CFTR-associated ligand, dJ94G16.2, FIGdJ94G16.2 PIST, Fused in glioblastoma, golgi-associated PDZ and coiled-coil motif containing, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC1, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, PISTGolgi associated PDZ and coiled-coil motif containing protein
Entrez Gene IDs
57120 (Human)
Gene Symbol
GOPC
UniProt
Additional PIST Products
Product Documents for PIST Antibody
Product Specific Notices for PIST Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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