Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all PIST Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human PIST. The immunogen is located within amino acids 360 - 410 of PIST. Amino Acid Squence: APEVDSDDENVEYEDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIST Antibody - BSA Free

Western Blot: PIST AntibodyBSA Free [NBP1-77242]

Western Blot: PIST AntibodyBSA Free [NBP1-77242]

Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.
PIST Antibody - BSA Free

Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] -

Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in human testis tissue with PIST antibody at 2 ug/ml.
PIST Antibody - BSA Free

Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] -

Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 ug/mL.
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Applications for PIST Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

1-4 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIST

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

Alternate Names

CALPDZ protein interacting specifically with TC10, CFTR-associated ligand, dJ94G16.2, FIGdJ94G16.2 PIST, Fused in glioblastoma, golgi-associated PDZ and coiled-coil motif containing, Golgi-associated PDZ and coiled-coil motif-containing protein, GOPC1, PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10, PISTGolgi associated PDZ and coiled-coil motif containing protein

Gene Symbol

GOPC

UniProt

AAG00572 (Human)

Additional PIST Products

Product Documents for PIST Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PIST Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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