PITX1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1546
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Paired-Like Homeodomain Transcription Factor 1 using the numbering given in entry NP_002644.3 (GeneID 5307).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PITX1 Antibody
![Western Blot: PITX1 Antibody [NB100-1546]](https://resources.bio-techne.com/images/products/PITX1-Antibody-Western-Blot-NB100-1546-img0003.jpg "Western Blot: PITX1 Antibody [NB100-1546]")
Western Blot: PITX1 Antibody [NB100-1546]
Western Blot: PITX1 Antibody [NB100-1546] - Detection of Human PITX by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PITX antibody NB100-1546 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
PITX1 Antibody [NB100-1546] - Detection of human PITX by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PITX antibody NB100-1546 (lot NB100-1546-2) used for IP at 6 ug per reaction. PITX was also immunoprecipitated by a previous lot of this antibody NB100-1546-1. For blotting immunoprecipitated PITX, A300-577A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Applications for PITX1 Antibody
Application
Recommended Usage
Immunoprecipitation
1-4 ug/mg of lysate
Western Blot
1:2500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PITX1
Alternate Names
BFTpituitary homeobox 1, CCF, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, paired-like homeodomain 1, pituitary otx-related factor, POTXpituitary homeo box 1, PTX1Paired-like homeodomain transcription factor 1hindlimb expressed homeobox protein backfoot
Entrez Gene IDs
5307 (Human)
Gene Symbol
PITX1
UniProt
Additional PITX1 Products
Product Documents for PITX1 Antibody
Product Specific Notices for PITX1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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