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PITX3 Antibody (28A9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26380

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26380-100ul
NBP3-26380-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 28A9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from Human PITX3 [UniProt O75364]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PITX3 Antibody (28A9)

PITX3 Antibody (28A9)

Immunocytochemistry/Immunofluorescence: PITX3 Antibody (28A9) [NBP3-26380] -

Immunocytochemistry/Immunofluorescence: PITX3 Antibody (28A9) [NBP3-26380] - Staining of A549 with PITX3 Antibody (28A9) at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 520-conjugated Goat Anti-Rabbit IgG (H+L).
PITX3 Antibody (28A9)

Flow Cytometry: PITX3 Antibody (28A9) [NBP3-26380] -

Flow Cytometry: PITX3 Antibody (28A9) [NBP3-26380] - Overlay Peak curve showing SH-SY5Y cells stained with PITX3 Antibody (28A9) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for PITX3 Antibody (28A9)

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: PITX3

PITX3 encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. Members of this family act as transcription factors. This protein is involved in lens formation during eye development. Mutations of this gene have been associated with anterior segment mesenchymal dysgenesis and congenital cataracts. [provided by RefSeq]

Alternate Names

CTPP4, Homeobox protein PITX3, MGC12766, paired-like homeodomain 3, Paired-like homeodomain transcription factor 3, pituitary homeobox 3, PTX3

Gene Symbol

PITX3

Additional PITX3 Products

Product Documents for PITX3 Antibody (28A9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PITX3 Antibody (28A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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