PIWIL4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19845

Novus Biologicals, part of Bio-Techne
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NBP2-19845
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIWIL4 Antibodies »

Product Summary for PIWIL4 Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PIWIL4. The exact sequence is proprietary.

Reactivity Notes

Rat (80%), Pig (85%).

Localization

Nucleus (By similarity); Cytoplasm (By similarity)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PIWIL4 Antibody

Western Blot: PIWIL4 Antibody [NBP2-19845]

Western Blot: PIWIL4 Antibody [NBP2-19845]

Western Blot: PIWIL4 Antibody [NBP2-19845] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PIWIL4 antibody [C2C3], C-term diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: PIWIL4 Antibody [NBP2-19845]

Immunocytochemistry/ Immunofluorescence: PIWIL4 Antibody [NBP2-19845]

Immunocytochemistry/Immunofluorescence: PIWIL4 Antibody [NBP2-19845] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: PIWIL4 Antibody [NBP2-19845]

Immunohistochemistry-Paraffin: PIWIL4 Antibody [NBP2-19845]

Immunohistochemistry-Paraffin: PIWIL4 Antibody [NBP2-19845] - Immunohistochemical analysis of paraffin-embedded 59T xenograft, using antibody at 1:500 dilution.

Applications for PIWIL4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PIWIL4

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM]

Alternate Names

HIWI2, MIWI2

Gene Symbol

PIWIL4

UniProt

Q7Z3Z4

Additional PIWIL4 Products

Product Documents for PIWIL4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PIWIL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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