PIWIL4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-49258

Novus Biologicals, part of Bio-Techne
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NBP2-49258
NBP2-49258-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PIWIL4 Antibodies »

Product Summary for PIWIL4 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GRARVKARGIARSPSATEVGRIQASPLPRSVDLSNNEASSSNGFLGTSRISTNDKYGISSGDAGSTFMERGVKNKQDFMDLSICTREKLAHVRNCKTGS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PIWIL4 Antibody

Western Blot: PIWIL4 Antibody [NBP2-49258]

Western Blot: PIWIL4 Antibody [NBP2-49258]

Western Blot: PIWIL4 Antibody [NBP2-49258] - Analysis in control (vector only transfected HEK293T lysate) and PIWIL4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: PIWIL4 Antibody [NBP2-49258]

Immunohistochemistry-Paraffin: PIWIL4 Antibody [NBP2-49258]

Immunohistochemistry-Paraffin: PIWIL4 Antibody [NBP2-49258] - Analysis in human testis and skeletal muscle tissues using NBP2-49258 antibody. Corresponding PIWIL4 RNA-seq data are presented for the same tissues.
Immunohistochemistry: PIWIL4 Antibody [NBP2-49258]

Immunohistochemistry: PIWIL4 Antibody [NBP2-49258]

Immunohistochemistry: PIWIL4 Antibody [NBP2-49258] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
View 1 more images

Applications for PIWIL4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PIWIL4

PIWIL4 belongs to the Argonaute family of proteins, which function in development and maintenance of germline stem cells (Sasaki et al., 2003 [PubMed 12906857]).[supplied by OMIM]

Alternate Names

HIWI2, MIWI2

Gene Symbol

PIWIL4

Additional PIWIL4 Products

Product Documents for PIWIL4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PIWIL4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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