PKA R2 Antibody (OTI1F8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73428

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PKA R2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PRKAR2A (NP_004148) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

45.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA R2 Antibody (OTI1F8) - Azide and BSA Free

Western Blot: PKA R2 Antibody (OTI1F8)Azide and BSA Free [NBP2-73428]

Western Blot: PKA R2 Antibody (OTI1F8)Azide and BSA Free [NBP2-73428]

Western Blot: PKA R2 Antibody (OTI1F8) - Azide and BSA Free [NBP2-73428] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKA R2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKA R2.
Immunocytochemistry/ Immunofluorescence: PKA R2 Antibody (OTI1F8) - Azide and BSA Free [NBP2-73428]

Immunocytochemistry/ Immunofluorescence: PKA R2 Antibody (OTI1F8) - Azide and BSA Free [NBP2-73428]

Immunocytochemistry/Immunofluorescence: PKA R2 Antibody (OTI1F8) - Azide and BSA Free [NBP2-73428] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PKA R2.
Immunohistochemistry: PKA R2 Antibody (OTI1F8) - Azide and BSA Free [NBP2-73428]

Immunohistochemistry: PKA R2 Antibody (OTI1F8) - Azide and BSA Free [NBP2-73428]

Immunohistochemistry: PKA R2 Antibody (OTI1F8) - Azide and BSA Free [NBP2-73428] - Staining of paraffin-embedded Human Kidney tissue using anti-PKA R2 mouse monoclonal antibody.
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Applications for PKA R2 Antibody (OTI1F8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA R2

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Alternate Names

protein kinase, cAMP-dependent, regulatory, type II, alpha, RII-alpha subunit

Gene Symbol

PRKAR2A

Additional PKA R2 Products

Product Documents for PKA R2 Antibody (OTI1F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKA R2 Antibody (OTI1F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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