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PKA R2 [p Ser99] Antibody (S05-7G2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19740

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-19740-100ul
NBP3-19740-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S05-7G2

Concentration

0.3 mg/ml

Product Summary for PKA R2 [p Ser99] Antibody (S05-7G2)

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Ser99 of human PKA R2 (Uniprot # P13861)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKA R2 [p Ser99] Antibody (S05-7G2)

Western Blot: PKA R2 [p Ser99] Antibody (S05-7G2) [NBP3-19740] - Western blot detection of Phospho-PKA R2 (Ser99) in Rat Brain, C6 cell lysates using NBP3-19740 (1:1000 diluted). Predicted band size: 46kDa. Observed band size: 50kDa.
Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (S05-7G2) [NBP3-19740] - Immunohistochemistry of PKA R2 (Phospho-Ser99) in paraffin-embedded Human tonsil using NBP3-19740 at dilution 1/100

Applications for PKA R2 [p Ser99] Antibody (S05-7G2)

Application
Recommended Usage

Immunohistochemistry

1/100

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKA R2

PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).

Alternate Names

protein kinase, cAMP-dependent, regulatory, type II, alpha, RII-alpha subunit

Gene Symbol

PRKAR2A

Additional PKA R2 Products

Product Documents for PKA R2 [p Ser99] Antibody (S05-7G2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKA R2 [p Ser99] Antibody (S05-7G2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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