PKC alpha Antibody (133)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07334
![Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334]](https://resources.bio-techne.com/images/products/PKC-alpha-Antibody-133-Immunohistochemistry-Paraffin-NBP3-07334-img0001.jpg "Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 133
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Full-length protein corresponding to PKC alpha. Recognizes sequence PQFVHPILQSAV at the C terminus at PKCalpha. (Uniprot: P17252)
Localization
Cytoplasm, Cell Membrane, Nucleus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
77 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08229)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for PKC alpha Antibody (133)
Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334]
Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).
Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] -
Immunohistochemistry-Paraffin: PKC alpha Antibody (133) [NBP3-07334] - Formalin-fixed, paraffin-embedded human tonsil stained with PKC alpha Mouse Monoclonal Antibody (133).Applications for PKC alpha Antibody (133)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: PKC alpha
Additional PKC alpha Products
Product Documents for PKC alpha Antibody (133)
Product Specific Notices for PKC alpha Antibody (133)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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