PKC alpha Antibody (M237)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23297
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # M237
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone (M237) was generated from a recombinant human PKC alpha that included amino acids residues in the central region. This region is highly conserved in rat and mouse PKC alpha, and has homology to conserved regions in PKC beta. [UniProt# P17252]
Specificity
This antibody detects an 82kDa protein corresponding to the molecular mass of PKC alpha on SDS-PAGE immunoblots of neonatal rat brain and adult mouse brain lysates.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for PKC alpha Antibody (M237)
Western Blot: PKC alpha Antibody (M237) [NBP3-23297] -
Western Blot: PKC alpha Antibody (M237) [NBP3-23297] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.Applications for PKC alpha Antibody (M237)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
6:00
Immunohistochemistry
1:500
Immunoprecipitation
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: PKC alpha
Long Name
Protein Kinase C alpha
Alternate Names
PKCA, PRKCA
Gene Symbol
PRKCA
Additional PKC alpha Products
Product Documents for PKC alpha Antibody (M237)
Product Specific Notices for PKC alpha Antibody (M237)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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