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PKC alpha [p Thr638] Antibody (S09-7C7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19548

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-19548-100ul
NBP3-19548-50ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S09-7C7

Concentration

0.3 mg/ml

Product Specifications

Immunogen

A synthetic phosphopeptide corresponding to residues surrounding Thr638 of human PKC alpha (Uniprot # P17252)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC alpha [p Thr638] Antibody (S09-7C7)

Western Blot: PKC alpha [p Thr638] Antibody (S09-7C7) [NBP3-19548] - Western blot detection of PKC alpha in K562, C6 cell lysates using NBP3-19548 (1:1000 diluted). Predicted band size: 77kDa. Observed band size: 80kDa.
Immunohistochemistry-Paraffin: PKC alpha [p Thr638] Antibody (S09-7C7) [NBP3-19548] - Immunohistochemistry of PKC alpha in paraffin-embedded human colon cancer tissue using NBP3-19548 at dilution 1/100

Applications for PKC alpha [p Thr638] Antibody (S09-7C7)

Application
Recommended Usage

Immunohistochemistry

1/50

Western Blot

1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC alpha

Protein Kinase C (PKC) is a large superfamily of serine/threonine kinases that mediate essential cellular signals required for activation, proliferation, differentiation and survival. There are at least ten PKC isotypes that are closely related in structure but that have distinct patterns of tissue distribution and function. The PKC isotypes can be subdivided into three classes based on primary structure and biochemical properties. These are: classical or conventional PKC isotypes (cPKC), novel PKC isotypes (nPKC) and atypical PKC isotypes (aPKC). All PKC isotypes share a characteristic equence motif C1 in addition to a serine/threonine-protein kinase domain. The cPKC isotypes include PKC

Long Name

Protein Kinase C alpha

Alternate Names

PKCA, PRKCA

Gene Symbol

PRKCA

Additional PKC alpha Products

Product Documents for PKC alpha [p Thr638] Antibody (S09-7C7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PKC alpha [p Thr638] Antibody (S09-7C7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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