PKC eta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38711

Novus Biologicals, part of Bio-Techne
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NBP2-38711-25ul
NBP2-38711
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC eta Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKC eta Antibody

Immunocytochemistry/ Immunofluorescence: PKC eta Antibody [NBP2-38711]

Immunocytochemistry/ Immunofluorescence: PKC eta Antibody [NBP2-38711]

Immunocytochemistry/Immunofluorescence: PKC eta Antibody [NBP2-38711] - Staining of human cell line MCF7 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PKC eta Antibody [NBP2-38711]

Immunohistochemistry-Paraffin: PKC eta Antibody [NBP2-38711]

Immunohistochemistry-Paraffin: PKC eta Antibody [NBP2-38711] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PKC eta Antibody [NBP2-38711]

Immunohistochemistry-Paraffin: PKC eta Antibody [NBP2-38711]

Immunohistochemistry-Paraffin: PKC eta Antibody [NBP2-38711] - Staining of human lymphoid tissues shows strong cytoplasmic positivity in germinal and non-germinal cells.
View 1 more images

Applications for PKC eta Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC eta

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene.

Long Name

Protein kinase C eta type

Alternate Names

EC 2.7.11.13, nPKC-eta, PKC-L, PKCL, PRKCH

Gene Symbol

PRKCH

UniProt

P24723 (Human)

Additional PKC eta Products

Product Documents for PKC eta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC eta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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