PKC gamma Antibody (A12-H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30124

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP1-30124-0.05ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # A12-H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC gamma Antibodies »

Product Summary for PKC gamma Antibody (A12-H)

Immunogen

Peptide derived from internal sequence of human PKC gamma. Antibody recognizes the epitope located between Leu405 - Pro414.

Epitope

Leu405 - Pro414

Specificity

This antibody is specific for PKC-gamma.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for PKC gamma Antibody (A12-H)

Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124]

Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124]

Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124] - Left Lane: Marker, Right Lane: Mouse brain crude lysate (200ug of protein loaded).
Immunocytochemistry/ Immunofluorescence: PKC gamma Antibody (A12-H) [NBP1-30124]

Immunocytochemistry/ Immunofluorescence: PKC gamma Antibody (A12-H) [NBP1-30124]

Immunocytochemistry/Immunofluorescence: PKC gamma Antibody (A12-H) [NBP1-30124] - PKC-gamma expression in HEK293 cells.

Applications for PKC gamma Antibody (A12-H)

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:2000
Application Notes
For western blots, incubate the membrane with antibody diluted in blocking buffer for 2 hours at room temperature. Western immunoblotting solutions: Wash buffer: 1x Tris Buffered Saline (TBS); 0.1% Triton X-100. Blocking buffer: 1xTBS; 0.1% Triton X-100; 5% nonfat dry milk.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC gamma

Protein Kinase C gamma (PKC gamma) is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and in regulating intercellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for its autophosphorylation on threonine 655 in the turn loop, and threonine 674 in the hydrophobic loop of the carboxy terminus, a critical step in generating a catalytically mature enzyme. The phosphorylation of the hydrophobic loop in the carboxyl terminus of PKCs is believed to be a key determinant in regulating PKC interaction with PDK1.

Long Name

Protein Kinase C gamma

Alternate Names

PKCC, PKCG, PRKCG, SCA14

Entrez Gene IDs

5582 (Human); 18752 (Mouse); 24681 (Rat)

Gene Symbol

PRKCG

UniProt

P05129, P63318 (Mouse), P63319 (Rat)

Additional PKC gamma Products

Product Documents for PKC gamma Antibody (A12-H)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PKC gamma Antibody (A12-H)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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