PKC iota Antibody (1A10)

Novus Biologicals, part of Bio-Techne | Catalog # H00005584-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1A10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC iota Antibodies »

Product Specifications

Immunogen

PRKCI (AAH22016, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSHTVAGGGSGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCV

Specificity

PRKCI (1A10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKC iota Antibody (1A10)

Western Blot: PKC iota Antibody (1A10) [H00005584-M03]

Western Blot: PKC iota Antibody (1A10) [H00005584-M03]

Western Blot: PKC iota Antibody (1A10) [H00005584-M03] - Analysis of PRKCI expression in Hela S3 NE (Cat # L013V3).
Sandwich ELISA: PKC iota Antibody (1A10) [H00005584-M03] - Detection limit for recombinant GST tagged PRKCI is approximately 0.03ng/ml as a capture antibody.

Applications for PKC iota Antibody (1A10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKC iota

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Long Name

Protein kinase C iota type

Alternate Names

aPKC-lambda/iota, DXS1179E, nPKC-iota, PRKC-lambda/iota

Entrez Gene IDs

5584 (Human)

Gene Symbol

PRKCI

OMIM

600539 (Human)

UniProt

AAH22016 (Human)

Additional PKC iota Products

Product Documents for PKC iota Antibody (1A10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC iota Antibody (1A10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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