PKC iota Antibody (1G11)

Novus Biologicals, part of Bio-Techne | Catalog # H00005584-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1G11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC iota Antibodies »

Product Specifications

Immunogen

PRKCI (AAH22016, 1 a.a. ~ 587 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSHTVAGGGSGDHSHQVRVKAYYRGDIMITHFEPSISFEGLCNEVRDMCSFDNEQLFTMKWIDEEGDPCTVSSQLELEEAFRLYELNKDSELLIHVFPCVPERPGMPCPGEDKSIYRRGARRWRKLYCANGHTFQAKRFNRRAHCAICTDRIWGLGRQGYKCINCKLLVHKKCHKLVTIECGRHSLPQEPVMPMDQSSMHSDHAQTVIPYNPSSHESLDQVGEEKEAMNTRESGKASSSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSMSVKAASVLKSFLNKDPKERLGCLPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNERVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV

Specificity

PRKCI - protein kinase C, iota

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PKC iota Antibody (1G11)

Western Blot: PKC iota Antibody (1G11) [H00005584-M01]

Western Blot: PKC iota Antibody (1G11) [H00005584-M01]

Western Blot: PKC iota Antibody (1G11) [H00005584-M01] - Analysis of PRKCI expression in transfected 293T cell line by PRKCI monoclonal antibody (M01), clone 1G11.Lane 1: PRKCI transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: PKC iota Antibody (1G11) [H00005584-M01]

Immunocytochemistry/ Immunofluorescence: PKC iota Antibody (1G11) [H00005584-M01]

Immunocytochemistry/Immunofluorescence: PKC iota Antibody (1G11) [H00005584-M01] - Analysis of monoclonal antibody to PRKCI on HeLa cell . Antibody concentration 10 ug/ml.
Western Blot: PKC iota Antibody (1G11) [H00005584-M01]

Western Blot: PKC iota Antibody (1G11) [H00005584-M01]

Western Blot: PKC iota Antibody (1G11) [H00005584-M01] - Analysis of PRKCI over-expressed 293 cell line, cotransfected with PRKCI Validated Chimera RNAi ( Cat # H00005584-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKCI monoclonal antibody (M01) clone 1G11 (Cat # H00005584-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
View 1 more images

Applications for PKC iota Antibody (1G11)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKC iota

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X.

Long Name

Protein kinase C iota type

Alternate Names

aPKC-lambda/iota, DXS1179E, nPKC-iota, PRKC-lambda/iota

Entrez Gene IDs

5584 (Human)

Gene Symbol

PRKCI

OMIM

600539 (Human)

UniProt

AAH22016 (Human)

Additional PKC iota Products

Product Documents for PKC iota Antibody (1G11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC iota Antibody (1G11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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