PKC theta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19851

Novus Biologicals, part of Bio-Techne
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NBP2-19851
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKC theta Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKC theta. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKC theta Antibody

Western Blot: PKC theta Antibody [NBP2-19851]

Western Blot: PKC theta Antibody [NBP2-19851]

Western Blot: PKC theta Antibody [NBP2-19851] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC theta antibody [N1N3-2] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PKC theta Antibody [NBP2-19851]

Immunohistochemistry-Paraffin: PKC theta Antibody [NBP2-19851]

Immunohistochemistry-Paraffin: PKC theta Antibody [NBP2-19851] - Mouse muscle. PKC theta antibody [N1N3-2] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: PKC theta Antibody [NBP2-19851]

Immunohistochemistry-Paraffin: PKC theta Antibody [NBP2-19851]

Immunohistochemistry-Paraffin: PKC theta Antibody [NBP2-19851] - C2C12 xenograft, using PKC theta antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
View 1 more images

Applications for PKC theta Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKC theta

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq]

Long Name

Protein Kinase C theta

Alternate Names

PRKCQ, PRKCT

Gene Symbol

PRKCQ

UniProt

Q04759

Additional PKC theta Products

Product Documents for PKC theta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PKC theta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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