PKC zeta Antibody (R14-K)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30127

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP1-30127-0.05ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # R14-K

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKC zeta Antibodies »

Product Specifications

Immunogen

Peptide derived from internal sequence on human PKC zeta. Antibody recognizes the epitope located between Lys220 - Leu231

Epitope

Lys220 - Leu231

Specificity

This antibody is specific for PKC-Zeta.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

Scientific Data Images for PKC zeta Antibody (R14-K)

Western Blot: PKC zeta Antibody (R14-K) [NBP1-30127]

Western Blot: PKC zeta Antibody (R14-K) [NBP1-30127]

Western Blot: PKC zeta Antibody (R14-K) [NBP1-30127] - Lane 1-50ng of recombinant human PKC-zeta (N-term GST PKC-zeta; BML-SE443-0005); lane 2-mouse brain crude protein lysate (100 ug); lane 3-rat brain crude.
Immunocytochemistry/ Immunofluorescence: PKC zeta Antibody (R14-K) [NBP1-30127]

Immunocytochemistry/ Immunofluorescence: PKC zeta Antibody (R14-K) [NBP1-30127]

Immunocytochemistry/Immunofluorescence: PKC zeta Antibody (R14-K) [NBP1-30127] - Expression in HEK293 cells.

Applications for PKC zeta Antibody (R14-K)

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:5000
Application Notes
Dilutions for IP must be determined by end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

20mM Tris/HCl (pH 8.0) and 10 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PKC zeta

Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.

Long Name

Protein Kinase C zeta

Alternate Names

PKC2, PRKCZ

Entrez Gene IDs

5590 (Human)

Gene Symbol

PRKCZ

Additional PKC zeta Products

Product Documents for PKC zeta Antibody (R14-K)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKC zeta Antibody (R14-K)

This antibody is immunoaffinity purified with immunogenic peptide as a ligand.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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