PKLR Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20027

Novus Biologicals, part of Bio-Techne
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NBP2-20027
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKLR Antibodies »

Product Summary for PKLR Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PKLR. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PKLR Antibody

Western Blot: PKLR Antibody [NBP2-20027]

Western Blot: PKLR Antibody [NBP2-20027]

Western Blot: PKLR Antibody [NBP2-20027] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKLR Antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-20027]

Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-20027]

Immunohistochemistry-Paraffin: PKLR Antibody [NBP2-20027] - Human breast cancer, using Pyruvate Kinase (liver/RBC) antibody at 1:250 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: PKLR Antibody [NBP2-20027]

Western Blot: PKLR Antibody [NBP2-20027]

Western Blot: PKLR Antibody [NBP2-20027] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 7.5% SDS PAGE diluted at 1:1000
View 1 more images

Applications for PKLR Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKLR

The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Pyruvate Kinase, Liver And RBC

Alternate Names

PK1, PKL, Pyruvate Kinase 1, RPK

Gene Symbol

PKLR

UniProt

P30613

Additional PKLR Products

Product Documents for PKLR Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PKLR Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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