PKM1 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14833CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-14833AF350

Alexa Fluor 405

NBP2-14833AF405

Alexa Fluor 488

NBP2-14833AF488

Alexa Fluor 532

NBP2-14833AF532

Alexa Fluor 594

NBP2-14833AF594

Alexa Fluor 647

NBP2-14833AF647

Alexa Fluor 700

NBP2-14833AF700

Alexa Fluor 750

NBP2-14833AF750

Allophycocyanin

NBP2-14833APC

Biotin

NBP2-14833B

DyLight 350

NBP2-14833UV

DyLight 405

NBP2-14833V

DyLight 488

NBP2-14833G

DyLight 550

NBP2-14833R

DyLight 594

NBP2-14833DL594

DyLight 650

NBP2-14833C

DyLight 680

NBP2-14833FR

DyLight 755

NBP2-14833IR

FITC

NBP2-14833F

HRP

NBP2-14833H

Janelia Fluor 525

NBP2-14833JF525

Janelia Fluor 549

NBP2-14833JF549

Janelia Fluor 646

NBP2-14833JF646

Janelia Fluor 669

NBP2-14833JF669

mFluor Violet 450 SE

NBP2-14833MFV450

mFluor Violet 500 SE

NBP2-14833MFV500

mFluor Violet 610 SE

NBP2-14833MFV610

PE

NBP2-14833PE

PerCP

NBP2-14833PCP

Forumulation

Catalog #

BSA Free

NBP2-14833

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Bovine

Applications

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PKM1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human PKM1 protein (between residues 350-450) [UniProt P14618-2].

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 25416385).

Localization

Cytoplasm.

Specificity

This antibody is specific for the M1 isoform of PKM.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for PKM1 Antibody [CoraFluor™ 1]

Application

Recommended Usage

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: PKM1

Pyruvate kinases are glycolytic enzymes that transfer a phosphate group from phosphoenolpyruvate to ADP generating pyruvate and ATP. There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues as well as in most cancer cells. The M1 isoform differs from the M2 isoform due to a change in sequence between residues 389-433. In the presence of D-fructose 1,6-bisphosphate (FBP) the monomer reversibly forms the highly active tetrameric form. This tetramer is associated with the glycolytic enzyme complex and activates the pyruvate kinase activity. A nearly inactive dimeric form is associates with tumor cells.

Alternate Names

PK1, Pyruvate kinase isozymes M1, Pyruvate kinase M1

Gene Symbol

PKM

Additional PKM1 Products

Product Documents for PKM1 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for PKM1 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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