PKMYT1 Antibody (2A3)

Novus Biologicals, part of Bio-Techne | Catalog # H00009088-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Proximity Ligation Assay, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2A3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PKMYT1 Antibodies »

Product Specifications

Immunogen

PKMYT1 (NP_004194.3, 400 a.a. ~ 499 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT

Specificity

PKMYT1 - protein kinase, membrane associated tyrosine/threonine 1 (2A3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for PKMYT1 Antibody (2A3)

Sandwich ELISA: PKMYT1 Antibody (2A3) [H00009088-M03] - Detection limit for recombinant GST tagged PKMYT1 is 1 ng/ml as a capture antibody.
Proximity Ligation Assay: PKMYT1 Antibody (2A3) [H00009088-M03]

Proximity Ligation Assay: PKMYT1 Antibody (2A3) [H00009088-M03]

Proximity Ligation Assay: PKMYT1 Antibody (2A3) [H00009088-M03] - Analysis of protein-protein interactions between CCNB1 and PKMYT1. Huh7 cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PKMYT1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for PKMYT1 Antibody (2A3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PKMYT1

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Alternate Names

DKFZp547K1610, EC 2.7.11.1, FLJ20093, Myt1 kinase, MYT1membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, protein kinase, membrane associated tyrosine/threonine 1

Entrez Gene IDs

9088 (Human)

Gene Symbol

PKMYT1

UniProt

NP_004194 (Human)

Additional PKMYT1 Products

Product Documents for PKMYT1 Antibody (2A3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PKMYT1 Antibody (2A3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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