PKNOX1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19853
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (91%), Mouse (94%), Rat (94%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human PKNOX1. The exact sequence is proprietary.
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PKNOX1 Antibody
![Western Blot: PKNOX1 Antibody [NBP2-19853]](https://resources.bio-techne.com/images/products/PKNOX1-Antibody-Western-Blot-NBP2-19853-img0004.jpg "Western Blot: PKNOX1 Antibody [NBP2-19853]")
Western Blot: PKNOX1 Antibody [NBP2-19853]
Western Blot: PKNOX1 Antibody [NBP2-19853] - HepG2 whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PKNOX1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: PKNOX1 Antibody [NBP2-19853]](https://resources.bio-techne.com/images/products/PKNOX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19853-img0002.jpg "Immunocytochemistry/ Immunofluorescence: PKNOX1 Antibody [NBP2-19853]")
Immunocytochemistry/ Immunofluorescence: PKNOX1 Antibody [NBP2-19853]
Immunocytochemistry/Immunofluorescence: PKNOX1 Antibody [NBP2-19853] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PKNOX1 protein stained by PKNOX1 antibody diluted at 1:1000. Blue: Hoechst 33342 staining.Applications for PKNOX1 Antibody
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
Assay dependent
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
Target validated by Mass Spectrometry
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PKNOX1
Alternate Names
Homeobox protein PREP-1, PBX/kn10Pbx regulating protein-1, pkonx1c
Gene Symbol
PKNOX1
UniProt
Additional PKNOX1 Products
Product Documents for PKNOX1 Antibody
Product Specific Notices for PKNOX1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.