PKR [p Thr449] Antibody (2F8)

Western Blot: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Positive WB detected in Hela whole cell lysate (treated with Calyculin A or not). All lanes PKR antibody at 1.25ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 62 KDa. Observed band size: 62 KDa.

Immunocytochemistry/Immunofluorescence: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - Immunofluorescence staining of Hela cells (treated with 50mM Calyculin A for 30 minutes) with at PKR antibody 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: PKR [p Thr449] Antibody (2F8) [NBP3-15204] - IHC image of PKR antibody diluted at 1:100 and staining in paraffin-embedded human kidney tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.