PLA2G2A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-42399

Novus Biologicals, part of Bio-Techne
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NBP2-42399
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all PLA2G2A Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-SYKFSNSGSRIT corresponding to internal region according to NP_000291.1.

Specificity

Reported variants represent identical protein: NP_001155199.1, NP_000291.1, NP_001155201.1, NP_001155200.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PLA2G2A Antibody

Western Blot: PLA2G2A Antibody [NBP2-42399]

Western Blot: PLA2G2A Antibody [NBP2-42399]

Western Blot: PLA2G2A Antibody [NBP2-42399] - Analysis of Human Placenta lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399]

Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399]

Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399] - Staining of paraffin embedded Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399]

Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399]

Immunohistochemistry-Paraffin: PLA2G2A Antibody [NBP2-42399] - Staining of paraffin embedded Human Liver. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for PLA2G2A Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:8000

Western Blot

0.1 - 0.3 ug/mL
Application Notes
WB: Approx. 19 kDa band observed in human placenta lysates (calculated MW of 16.1 kDa band according to NP_000291.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLA2G2A

The protein encoded by the Phospholipase A2 IIA gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.

Long Name

Phospholipase A2 Group IIA

Alternate Names

Mom1, PLA2B, PLA2L, RASF-A

Entrez Gene IDs

5320 (Human)

Gene Symbol

PLA2G2A

Additional PLA2G2A Products

Product Documents for PLA2G2A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLA2G2A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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