PLA2R1 Antibody (12-6-5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-50248

Novus Biologicals, part of Bio-Techne
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NBP2-50248
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 12-6-5

Format

BSA Free

Concentration

1 mg/ml

View all PLA2R1 Antibodies »

Product Specifications

Immunogen

Human PLA2R extracellular sequence N-C3 (containing domains N-terminal Cysteine rich-FibII-CTLD1-CTLD2-CTLD3). Genbank acc no. U17033; amino acids 20-663 Recombinant protein expressed in HEK 293-EBNA-1 cells

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for PLA2R1 Antibody (12-6-5) - BSA Free

Western Blot: PLA2R1 Antibody (12-6-5)BSA Free [NBP2-50248]

Western Blot: PLA2R1 Antibody (12-6-5)BSA Free [NBP2-50248]

Western Blot: PLA2R1 Antibody (12-6-5) [NBP2-50248] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000.
Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (12-6-5) - BSA Free [NBP2-50248]

Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (12-6-5) - BSA Free [NBP2-50248]

Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Staining of PLA2R over-expressing podocytes revealing cell surface expression of the PLA2R receptor. Dilution 1:400 (under 40x lens).
Immunohistochemistry: PLA2R1 Antibody (12-6-5) - BSA Free [NBP2-50248]

Immunohistochemistry: PLA2R1 Antibody (12-6-5) - BSA Free [NBP2-50248]

Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Membranous nephropathy kidney showing staining of PLA2R in the capillary wall of the glomerular basement membrane. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.
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Applications for PLA2R1 Antibody (12-6-5) - BSA Free

Application
Recommended Usage

ELISA

1:5000 - 1:20000

Flow Cytometry

1:200

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:5000 - 1:20000

Western Blot

1:5000 - 1:10000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLA2R1

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Gene Symbol

PLA2R1

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody (12-6-5) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PLA2R1 Antibody (12-6-5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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