PLA2R1 Antibody (CL0474)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52933

Novus Biologicals, part of Bio-Techne
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NBP2-52933
NBP2-52933-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0474

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLA2R1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: EEKTWHEALRSCQADNSALIDITSLAEVEFLVTLLGDENASETWIGLSSNKIPVSFEWSNDSSVIFTNWHTLEPHIFPNRSQLCVSAEQSEGHWKVKNCEERLFYICKKAGHVLSDAESGCQEGWERHGGFCYKID

Epitope

KIPVSFEWSN

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PLA2R1 Antibody (CL0474)

Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunohistochemistry-Paraffin: PLA2R1 Antibody (CL0474) [NBP2-52933] - Staining in human kidney and pancreas tissues. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
Western Blot: PLA2R1 Antibody (CL0474) [NBP2-52933]

Western Blot: PLA2R1 Antibody (CL0474) [NBP2-52933]

Western Blot: PLA2R1 Antibody (CL0474) [NBP2-52933] - Analysis in human kidney tissue.
Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933]

Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (CL0474) [NBP2-52933] - Enhanced validation of the Anti-PLA2R1 monoclonal antibody by comparing the immunofluorescence staining in human cell lines Hela and MCF7 exhibiting a relatively high and low expression of PLA2R1 (based on RNA-seq values), respectively. The PLA2R1 (green signal) is present in Hela cells but is absent in MCF7 cells. The anti-HDAC1 antibody was used as a loading control (red signal). Antibody staining is shown in green.
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Applications for PLA2R1 Antibody (CL0474)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20000 - 1:50000

Immunohistochemistry-Paraffin

1:20000 -1:50000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLA2R1

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Gene Symbol

PLA2R1

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody (CL0474)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PLA2R1 Antibody (CL0474)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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