PLAG1 Antibody (3B7)
Novus Biologicals, part of Bio-Techne | Catalog # H00005324-M02
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3B7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PLAG1 (NP_002646, 2 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ATVIPGDLSEVRDTQKVPSGKRKRGETKPRKNFPCQLCDKAFNSVEKLKVHSYSHTGERPYKCIQQDCTKAFVSKYKLQRHMATHSPEKTHKCNYCEK
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PLAG1 Antibody (3B7)
Detection of PLAG1 in Western Blot
Western Blot detection against Immunogen (36.52 KDa) .
Immunohistochemical Staining of Tumor Sections Using PLAG1 Antibody
Left, IHC showing TGFBR3 staining in whole tumor sections (upper panels, scale bars=5mm) and enlargement of selected areas (lower panels, scale bars=100um). Right, quantification of IHC TGFBR3 levels in TGFBR3-PLAG1 positive vs. negative tumors. P (high TGFBR3)=0.002, Fisher's exact test. n=5+34. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-017-01178-z), licensed under a CC-BY license.
Determination of Detection Limit for PLAG1 Antibody as Capture Antibody in ELISA
Detection limit for recombinant GST tagged PLAG1 is approximately 0.03ng/ml as a capture antibody.Applications for PLAG1 Antibody (3B7)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. IHC usage was reported in scientific literature.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PLAG1
Alternate Names
COL1A2/PLAG1 fusion, pleiomorphic adenoma gene 1, Pleiomorphic adenoma gene 1 protein, SGPA, zinc finger protein PLAG1, ZNF912
Entrez Gene IDs
5324 (Human)
Gene Symbol
PLAG1
OMIM
181030 (Human)
UniProt
Additional PLAG1 Products
Product Documents for PLAG1 Antibody (3B7)
Product Specific Notices for PLAG1 Antibody (3B7)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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