Plakophilin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90042

Novus Biologicals, part of Bio-Techne
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NBP1-90042
NBP1-90042-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Plakophilin 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YTVRNLMASQPQLAKQYFSSSMLNNIINLCRSSASPKAAEAARLLLSDMWSSKELQGVLRQQGFDRNMLGTLAGANSLRNFTSRF

Reactivity Notes

Mouse (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Plakophilin 1 Antibody

Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042]

Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042]

Immunohistochemistry-Paraffin: Plakophilin 1 Antibody [NBP1-90042] - Staining in human skin and skeletal muscle tissues using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are presented for the same tissues.
Western Blot: Plakophilin 1 Antibody [NBP1-90042]

Western Blot: Plakophilin 1 Antibody [NBP1-90042]

Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis in human cell lines PC-3 and A-549 using anti-PKP1 antibody. Corresponding PKP1 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.
Western Blot: Plakophilin 1 Antibody [NBP1-90042]

Western Blot: Plakophilin 1 Antibody [NBP1-90042]

Western Blot: Plakophilin 1 Antibody [NBP1-90042] - Analysis using Anti-PKP1 antibody NBP1-90042 (A) shows similar pattern to independent antibody NBP1-90041 (B).
View 4 more images

Applications for Plakophilin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Plakophilin 1

The Plakophilin 1 gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the

Alternate Names

B6PBand 6 protein, MGC138829, plakophilin 1 (ectodermal dysplasia/skin fragility syndrome), plakophilin-1

Gene Symbol

PKP1

Additional Plakophilin 1 Products

Product Documents for Plakophilin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Plakophilin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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