Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 23E3/4

Format

BSA Free

Concentration

1 mg/ml

View all Plakophilin-3 Antibodies »

Product Specifications

Immunogen

This antibody is a mouse monoclonal IgG2b antibody derived by fusion of Mouse myeloma cells with spleen cells from a Mouse immunized with a synthetic peptide corresponding to amino acid residues 570-589 of Human plakophilin-3 coupled to keyhole limpet hemocyanin via an additional cysteine residue at the N-terminus (CFTPQSRRLRELPLAADALTF).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This antibody reacts with an epitope located between residues 570-589 in Human plakophilin-3.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Plakophilin-3 Antibody (23E3/4) - BSA Free

Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) - BSA Free [NBP1-97674]

Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) - BSA Free [NBP1-97674]

Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) [NBP1-97674] - IHC localization of plakophilin-3 in hair root sheath of dog skin.
Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) - BSA Free [NBP1-97674]

Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) - BSA Free [NBP1-97674]

Immunohistochemistry: Plakophilin-3 Antibody (23E3/4) [NBP1-97674] - IHC localization of plakophilin-3 in hair root sheath of dog skin.

Applications for Plakophilin-3 Antibody (23E3/4) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:25-1:200

Immunohistochemistry-Paraffin

1:25-1:200

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:1000
Application Notes
This antibody is suitable for immunocytochemistry (methanol- and paraformaldehyde-fixed cells), immunohistochemistry on frozen and paraffin-embedded tissues, immunoprecipitation and Western blot detection. For optimal results, fixed cells and frozen tissues should be treated with 0.2% Triton-X 100 for, respectively, 5 min and 15 min prior to incubation with primary antibody. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Plakophilin-3

Plakophilin-3 is an Armadillo-like protein present in nuclei and desmosomes of epithelial cells. The expression pattern of this protein seems to be largely restricted to epithelial cell types. Plakophilin-3 can be detected along cell borders in a punctuate staining pattern typical for desmosomal proteins. In addition to the desmosomal immunolocalisation, immunostaining was observed as bright nuclear speckles. Thus, like plakophilin-1 and-2, plakophilin-3 displays a dual intracellular localisation in the desmosomal plaque and in the cell nucleus, and therefore is probably involved in signal transduction pathways between the plasma membrane and the nucleus. The Human protein has a predicted molecular mass of 87 kD.

Alternate Names

plakophilin 3, plakophilin-3

Gene Symbol

PKP3

Additional Plakophilin-3 Products

Product Documents for Plakophilin-3 Antibody (23E3/4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Plakophilin-3 Antibody (23E3/4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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