Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Plakophilin 4 Antibodies »

Product Summary for Plakophilin 4 Antibody

Immunogen

This antibody maps to a region between residue 1 to 50 of human Plakophilin 4 using the numbering given in entry NP_003619.2 (GeneID 8502).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Plakophilin 4 Antibody

Western Blot: Plakophilin 4 Antibody [NBP2-37691]

Western Blot: Plakophilin 4 Antibody [NBP2-37691]

Western Blot: Plakophilin 4 Antibody [NBP2-37691] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat and mouse TCMK-1 cells prepared using NETN lysis buffer. Affinity purified rabbit anti-p0071 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for Plakophilin 4 Antibody

Application
Recommended Usage

Western Blot

1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. Immunoprecipitation Not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Plakophilin 4

Plakophilin 4, also known by its gene name PKP4, has multiple transcript variants, however only two have been fully characterized. Isoform 1 has 1,192 amino acids and is approximately 132 kDa and the shorter 1,149 amino acid long isoform is approximately 127 kDa. Plakophilin 4 is a member of the Beta Catenin family and the Plakophilin subfamily of Armadillo-like proteins. Plakophilin 4 regulates Rho activity during cytokinesis and may be implicated in the regulation of junctional plaques. Current research on Plakophilin 4 is being performed relating to several diseases and disorders including Alzheimer's Disease, schizophrenia, renal cell carcinoma and cardiomyopathy. PKP4 interacts with Presenilin 1, Erbin, OSGEP, Desmoplakin and GABARAP.

Alternate Names

catenin 4, FLJ42243, p0071FLJ31261, plakophilin 4, plakophilin-4

Gene Symbol

PKP4

UniProt

NP_003619.2 (Human)

Additional Plakophilin 4 Products

Product Documents for Plakophilin 4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Plakophilin 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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