PLC-gamma 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38317

Novus Biologicals, part of Bio-Techne
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NBP2-38317
NBP2-38317-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLC-gamma 1 Antibodies »

Product Summary for PLC-gamma 1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLC-gamma 1 Antibody

Western Blot: PLC-gamma 1 Antibody [NBP2-38317]

Western Blot: PLC-gamma 1 Antibody [NBP2-38317]

Western Blot: PLC-gamma 1 Antibody [NBP2-38317] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: PLC-gamma 1 Antibody [NBP2-38317]

Immunocytochemistry/ Immunofluorescence: PLC-gamma 1 Antibody [NBP2-38317]

Immunocytochemistry/Immunofluorescence: PLC-gamma 1 Antibody [NBP2-38317] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Immunohistochemistry: PLC-gamma 1 Antibody [NBP2-38317]

Immunohistochemistry: PLC-gamma 1 Antibody [NBP2-38317]

Immunohistochemistry: PLC-gamma 1 Antibody [NBP2-38317] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.

Applications for PLC-gamma 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLC-gamma 1

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC-gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC-gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC-gamma in the physiological agonist-induced activation of Ca2+ entry (3).

Long Name

Phospholipase C gamma 1

Alternate Names

PLCG1, PLCgamma 1, PPLCA

Gene Symbol

PLCG1

UniProt

P19174

Additional PLC-gamma 1 Products

Product Documents for PLC-gamma 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLC-gamma 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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