PLD1 Antibody (2F3)
Novus Biologicals, part of Bio-Techne | Catalog # H00005337-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 2F3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
PLD1 (NP_002653, 965 a.a. ~ 1074 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT
Specificity
PLD1 - phospholipase D1, phophatidylcholine-specific
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for PLD1 Antibody (2F3)
![Western Blot: PLD1 Antibody (2F3) [H00005337-M01]](https://resources.bio-techne.com/images/products/PLD1-Antibody-2F3-Western-Blot-H00005337-M01-img0003.jpg "Western Blot: PLD1 Antibody (2F3) [H00005337-M01]")
Western Blot: PLD1 Antibody (2F3) [H00005337-M01]
Western Blot: PLD1 Antibody (2F3) [H00005337-M01] - Analysis of PLD1 expression in transfected 293T cell line by PLD1 monoclonal antibody (M01), clone 2F3.Lane 1: PLD1 transfected lysate(124.2 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: PLD1 Antibody (2F3) [H00005337-M01] - Detection limit for recombinant GST tagged PLD1 is 0.03 ng/ml as a capture antibody.
Applications for PLD1 Antibody (2F3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PLD1
Long Name
Phospholipase D1
Alternate Names
Choline phosphatase 1
Entrez Gene IDs
5337 (Human)
Gene Symbol
PLD1
OMIM
602382 (Human)
UniProt
Additional PLD1 Products
Product Documents for PLD1 Antibody (2F3)
Product Specific Notices for PLD1 Antibody (2F3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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