PLD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34078

Novus Biologicals, part of Bio-Techne
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NBP2-34078
NBP2-34078-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all PLD1 Antibodies »

Product Summary for PLD1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDVSPSDPKIQEVYIPFSAIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSINLYTIELTHGEFKWQVKRKFKHFQEFHR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLD1 Antibody

Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078]

Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078]

Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] - Staining in human gallbladder and skeletal muscle tissues using anti-PLD1 antibody. Corresponding PLD1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: PLD1 Antibody [NBP2-34078]

Immunocytochemistry/ Immunofluorescence: PLD1 Antibody [NBP2-34078]

Immunocytochemistry/Immunofluorescence: PLD1 Antibody [NBP2-34078] - Staining of human cell line U-2 OS shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078]

Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078]

Immunohistochemistry-Paraffin: PLD1 Antibody [NBP2-34078] - Staining of human skeletal muscle shows low expression as expected.
View 4 more images

Applications for PLD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLD1

Phosphatidylcholine (PC)-specific phospholipases D (PLDs; EC 3.1.4.4) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. A range of agonists acting through G protein-coupled receptors and receptor tyrosine kinases stimulate this hydrolysis. PC-specific PLD activity has been implicated in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis (Hammond et al., 1995 [PubMed 8530346]).[supplied by OMIM]

Long Name

Phospholipase D1

Alternate Names

Choline phosphatase 1

Gene Symbol

PLD1

UniProt

Q13393

Additional PLD1 Products

Product Documents for PLD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PLD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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