PLD3/Phospholipase D3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-35842
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 279-490 of human PLD3/Phospholipase D3 (NP_001026866).
Sequence:
MEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYMVTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL
Sequence:
MEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYMVTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PLD3/Phospholipase D3 Antibody
Immunocytochemistry/ Immunofluorescence: PLD3/Phospholipase D3 Antibody [NBP3-35842] -
Immunocytochemistry/ Immunofluorescence: PLD3/Phospholipase D3 Antibody [NBP3-35842] - Immunofluorescence analysis of L929 cells using PLD3/Phospholipase D3 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western Blot: PLD3/Phospholipase D3 Antibody [NBP3-35842] -
Western Blot: PLD3/Phospholipase D3 Antibody [NBP3-35842] - Western blot analysis of various lysates using PLD3/Phospholipase D3 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
Applications for PLD3/Phospholipase D3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Preservative
0.01% Thimerosal
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: PLD3/Phospholipase D3
Alternate Names
Choline phosphatase 3, EC 3.1.4, EC 3.1.4.4, HindIII K4L homolog, HUK4, Hu-K4, Phosphatidylcholine-hydrolyzing phospholipase D3, phospholipase D family, member 3, phospholipase D3, PLD 3
Gene Symbol
PLD3
Additional PLD3/Phospholipase D3 Products
Product Documents for PLD3/Phospholipase D3 Antibody
Product Specific Notices for PLD3/Phospholipase D3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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