PLD4/Phospholipase D4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13770
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Flow Cytometry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: AQDLEKTFQTYWVLGVPKAVLPKTWPQNFSSHFNRFQPFHGLFDGVPTTAYFSASPPALCPQGRTRDLEALLAVMGSAQEFIYASVMEYFP
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (81%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PLD4/Phospholipase D4 Antibody
![Western Blot: PLD4/Phospholipase D4 Antibody [NBP2-13770]](https://resources.bio-techne.com/images/products/PLD4-Phospholipase-D4-Antibody-Western-Blot-NBP2-13770-img0004.jpg "Western Blot: PLD4/Phospholipase D4 Antibody [NBP2-13770]")
Western Blot: PLD4/Phospholipase D4 Antibody [NBP2-13770]
Western Blot: PLD4/Phospholipase D4 Antibody [NBP2-13770] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue![Immunohistochemistry-Paraffin: PLD4/Phospholipase D4 Antibody [NBP2-13770]](https://resources.bio-techne.com/images/products/PLD4-Phospholipase-D4-Antibody-Immunohistochemistry-Paraffin-NBP2-13770-img0001.jpg "Immunohistochemistry-Paraffin: PLD4/Phospholipase D4 Antibody [NBP2-13770]")
Immunohistochemistry-Paraffin: PLD4/Phospholipase D4 Antibody [NBP2-13770]
Immunohistochemistry-Paraffin: PLD4/Phospholipase D4 Antibody [NBP2-13770] - Staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.Applications for PLD4/Phospholipase D4 Antibody
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PLD4/Phospholipase D4
Alternate Names
C14orf175, Choline phosphatase 4, chromosome 14 open reading frame 175, EC 3.1.4.4, Phosphatidylcholine-hydrolyzing phospholipase D4, phospholipase D family, member 4, phospholipase D4, PLD 4
Gene Symbol
PLD4
Additional PLD4/Phospholipase D4 Products
Product Documents for PLD4/Phospholipase D4 Antibody
Product Specific Notices for PLD4/Phospholipase D4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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