Plectin Antibody (7A8)
Novus Biologicals, part of Bio-Techne | Catalog # NB120-11220
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Mammal
Cited:
Bovine
Applications
Validated:
Dot Blot, Electron Microscopy, ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7A8
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Plectin purified from rat glioma C6 cells.
Epitope
Monoclonal Anti-Plectin recognizes an epitope located in the middle section on the rod domain of the plectin molecule, approximately half way between the globular end domains. The kinase C phosphorylation site was found on the same terminal segment as thi
Reactivity Notes
Bovine reactivity reported in scientific literature (PMID: 30591979).Cross-reacts with Marsupials. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Specificity
The inhibits the interaction of plectin with vimentin and lamin B.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Plectin Antibody (7A8)
![Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220]](https://resources.bio-techne.com/images/products/Plectin-Antibody-7A8-Immunohistochemistry-NB120-11220-img0001.jpg "Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220]")
Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220]
Immunohistochemistry: Plectin Antibody (7A8) [NB120-11220] - Frozen sections acetone fixed Rat cardiac muscle tissue sections were stained with 1:200 Monoclonal Anti-Plectin antibody produced in Mouse, Clone: 7A8, followed by 1:30 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat.Applications for Plectin Antibody (7A8)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
WB: Detects a band of approximately 300 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Preservative
0.1% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Plectin
Alternate Names
EBS1, HD1, Hemidesmosomal protein 1, LGMD2Q, PCNplectin 1, intermediate filament binding protein, 500kD, PLEC1b, PLEC1EBSO, plectin, plectin 1, intermediate filament binding protein 500kDa, plectin-1, PLTNepidermolysis bullosa simplex 1 (Ogna)
Entrez Gene IDs
64204 (Rat)
Gene Symbol
PLEC
Additional Plectin Products
Product Documents for Plectin Antibody (7A8)
Product Specific Notices for Plectin Antibody (7A8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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