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PLEKHA7 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85045

Novus Biologicals, part of Bio-Techne
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NBP1-85045
NBP1-85045-25ul

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SSLKRDMEKVERQAVPQANHTESCHECGRVGPGHTRDCPHRGHDDIVNFERQEQEGEQYRSQRDPLEGKRDRSKARSPYSPAEEDALFMDLPTGP

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23990464).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLEKHA7 Antibody

Western Blot: PLEKHA7 Antibody [NBP1-85045]

Western Blot: PLEKHA7 Antibody [NBP1-85045]

Western Blot: PLEKHA7 Antibody [NBP1-85045] - Analysis in control (vector only transfected HEK293T lysate) and PLEKHA7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: PLEKHA7 Antibody [NBP1-85045]

Immunocytochemistry/ Immunofluorescence: PLEKHA7 Antibody [NBP1-85045]

Immunocytochemistry/Immunofluorescence: PLEKHA7 Antibody [NBP1-85045] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & cell junctions.
Immunohistochemistry-Paraffin: PLEKHA7 Antibody [NBP1-85045]

Immunohistochemistry-Paraffin: PLEKHA7 Antibody [NBP1-85045]

Immunohistochemistry-Paraffin: PLEKHA7 Antibody [NBP1-85045] - Staining of human prostate shows moderate to strong cytoplasmic positivity in smooth muscle cells.

Applications for PLEKHA7 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
WB, ICC/IF reported in scientific literature (PMID: 23990464). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLEKHA7

Alternate Names

DKFZp686M22243, PH domain-containing family A member 7, pleckstrin homology domain containing, family A member 2, pleckstrin homology domain containing, family A member 7, pleckstrin homology domain-containing family A member 7

Gene Symbol

PLEKHA7

Additional PLEKHA7 Products

Product Documents for PLEKHA7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for PLEKHA7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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