Plexin A1 Antibody (M535)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23316
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M535
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone M535 was generated from a Plexin A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin A1. This sequence is highly conserved in rat and mouse Plexin A1, and has low homology to other Plexin family members. [UniProt# Q9UIW2]
Specificity
The antibody detects a 220 kDa protein corresponding to the apparent molecular mass of Plexin A1 on SDS-PAGE immunoblots of neonatal mouse brain, and detects a recombinant mouse Plexin A1 protein containing the extracellular region.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Plexin A1 Antibody (M535)
Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] -
Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).
Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (M535) [NBP3-23316] -
Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (M535) [NBP3-23316] - Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (NBP3-23316). The antibody was detected using goat anti-mouse DyLight(R) 594.Applications for Plexin A1 Antibody (M535)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100
Immunoprecipitation
1:50
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Plexin A1
Alternate Names
NOVP, PLXN1, PLXNA1
Gene Symbol
PLXNA1
Additional Plexin A1 Products
Product Documents for Plexin A1 Antibody (M535)
Product Specific Notices for Plexin A1 Antibody (M535)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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