Plexin A1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23309
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Plexin A1 Antibody
Immunogen
Plexin A1 synthetic peptide (coupled to carrier protein) corresponding to a region within the N-terminal half of the semaphorin domain in human Plexin A1. This sequence is highly conserved in rat and mouse Plexin A1, and has low homology to other Plexin family members. [UniProt# Q9UIW2]
Specificity
The antibody detects a 220 kDa protein corresponding to the apparent molecular mass of Plexin A1 on SDS-PAGE immunoblots of neonatal rat brain and Cos-7 cells transfected with mouse Plexin A1. In immunocytochemistry, this antibody co-localizes with anti-Myc in Cos-7 cells transfected with Myc-tagged mouse Plexin A1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Plexin A1 Antibody
Western Blot: Plexin A1 Antibody [NBP3-23309] -
Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).
Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] -
Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] - Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin A1 rabbit polyclonal (NBP3-23309) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of this anti-Plexin A1 antibody (C,F).Applications for Plexin A1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:100
Immunoprecipitation
1:50
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Plexin A1
Alternate Names
NOVP, PLXN1, PLXNA1
Gene Symbol
PLXNA1
Additional Plexin A1 Products
Product Documents for Plexin A1 Antibody
Product Specific Notices for Plexin A1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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