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Plexin B1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23325

Novus Biologicals, part of Bio-Techne
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NBP3-23325

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Plexin B1 Antibody

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Plexin B1. This sequence has high homology with similar regions in rat and mouse Plexin B1, and has low homology to other plexin family members. [UniProt# O43157]

Specificity

The antibody detects 300, 150, and 100 kDa bands on SDS-PAGE immunoblots of mouse liver and rat PC12 cells. These bands correspond to the full length and proteolytically processed forms of Plexin B1. In mouse liver, this antibody detects 300 and 100 kDa forms of Plexin B1.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Plexin B1 Antibody

Plexin B1 Antibody

Western Blot: Plexin B1 Antibody [NBP3-23325] -

Western Blot: Plexin B1 Antibody [NBP3-23325] - Western blot of mouse liver (lane 1) and rat PC12 cells (lane 2). The blot was probed with rabbit polyclonal Plexin B1 antibody (NBP3-23325) at a dilution of 1:1000.
Plexin B1 Antibody

Immunocytochemistry/Immunofluorescence: Plexin B1 Antibody [NBP3-23325] -

Immunocytochemistry/Immunofluorescence: Plexin B1 Antibody [NBP3-23325] - Immunocytochemical labeling in rat PC12 cells differentiated with NGF. The cells were probed with rabbit polyclonal Plexin B1 (NBP3-23325) and mouse monoclonal Sema-4D antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.

Applications for Plexin B1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Plexin B1

Receptor for SEMA4D. Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration

Alternate Names

PLXN5, PLXNB1, Transmembrane Protein SEP

Gene Symbol

PLXNB1

Additional Plexin B1 Products

Product Documents for Plexin B1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Plexin B1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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