Plexin D1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23326

Novus Biologicals, part of Bio-Techne
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NBP3-23326
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

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Product Specifications

Immunogen

Plexin D1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the cytoplasmic domain from human Plexin D1. This sequence is well conserved in rat and mouse Plexin D1, and has low homology to other Plexin family members. [UniProt# Q9Y4D7]

Specificity

The antibody detects 250, 215, and 195 kDa bands corresponding to the apparent molecular mass of Plexin D1 variants in human umbilical vein endothelial cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

195 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Plexin D1 Antibody

Plexin D1 Antibody

Western Blot: Plexin D1 Antibody [NBP3-23326] -

Western Blot: Plexin D1 Antibody [NBP3-23326] - Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).

Applications for Plexin D1 Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Plexin D1

Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors. Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins. The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and approximately 84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron. Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus. Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct. Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities. Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development. In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal. Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E. Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands.

Alternate Names

PLXND1

Gene Symbol

PLXND1

Additional Plexin D1 Products

Product Documents for Plexin D1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Plexin D1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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