PLK1 [p Thr210] Antibody (8G9X10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16917

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16917-100ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8G9X10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PLK1 Antibodies »

Product Summary for PLK1 [p Thr210] Antibody (8G9X10)

Immunogen

A synthetic phosphorylated peptide around T210 of human PLK1 (P53350). KKTLC

Modification

p Thr 210

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PLK1 [p Thr210] Antibody (8G9X10)

Western Blot: PLK1 [p Thr210] Antibody (8G9X10) [NBP3-16917] - Western blot analysis of extracts of HT-29 cells, using PLK1 (NBP3-16917) at 1:1000 dilution.HT-29 cells were treated by nocodazole (100 ng/mL) at 37C for 16 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for PLK1 [p Thr210] Antibody (8G9X10)

Application
Recommended Usage

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLK1

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Gene Symbol

PLK1

Additional PLK1 Products

Product Documents for PLK1 [p Thr210] Antibody (8G9X10)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PLK1 [p Thr210] Antibody (8G9X10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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