PLRG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06556

Novus Biologicals, part of Bio-Techne
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NBP1-06556
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all PLRG1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 464 and 514 of human pleiotropic regulator 1 using the numbering given in entry NP_002660.1 (GeneID 5356).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLRG1 Antibody

Western Blot: PLRG1 Antibody [NBP1-06556]

Western Blot: PLRG1 Antibody [NBP1-06556]

Western Blot: PLRG1 Antibody [NBP1-06556] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. NBP1-06556 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate.
Immunocytochemistry/ Immunofluorescence: PLRG1 Antibody [NBP1-06556]

Immunocytochemistry/ Immunofluorescence: PLRG1 Antibody [NBP1-06556]

Immunocytochemistry/Immunofluorescence: PLRG1 Antibody [NBP1-06556] - human breast carcinoma. Antibody dilution of 1:100.
Immunohistochemistry: PLRG1 Antibody [NBP1-06556]

Immunohistochemistry: PLRG1 Antibody [NBP1-06556]

Immunohistochemistry: PLRG1 Antibody [NBP1-06556] - Sections of human prostate carcinoma (left) and breast carcinoma (right). Antibody: Affinity purified rabbit anti- PLRG1 used at a dilution of 1:200 (1ug/ml). Detection: DAB and Redfluorescent goat anti-rabbit IgG-heavy and light chain,cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1:100

Applications for PLRG1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NBP1-06556 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PLRG1

Pleiotropic regulator 1 (PLRG1) has been shown to be a part of sub-spliceosomal protein complexes. It is highly conserved across species and is essential for pre-mRNA splicing. PLRG1 interacts with human CDC5. This interaction has also been shown to be essential to pre-mRNA splicing. PLRG1 is also known as PRL1, and MGC110980.

Alternate Names

MGC110980, pleiotropic regulator 1 (PRL1 homolog, Arabidopsis), PRL1Arabidopsis homolog)

Entrez Gene IDs

5356 (Human); 53317 (Mouse)

Gene Symbol

PLRG1

UniProt

O43660 (Human)

Additional PLRG1 Products

Product Documents for PLRG1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PLRG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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