PMCA4 Antibody (JA9)

Novus Biologicals, part of Bio-Techne | Catalog # NB300-569

Novus Biologicals, part of Bio-Techne
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NB300-569
NB300-569-0.025mg
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Amphibian, Bovine, Rabbit

Cited:

Human, Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # JA9

Concentration

1 mg/ml

View all PMCA4 Antibodies »

Product Specifications

Immunogen

Purified human erythrocyte PMCA.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 23845592). Rabbit reactivity reported in scientific literature (PMID: 21139678). Bovine reactivity reported in scientific literature (PMID: 23715721). Amphibian reactivity reported in scientific literature (PMID: 15236230). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This is specific to the PMCA4 isoform.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PMCA4 Antibody (JA9)

Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569]

Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569]

Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Immunolocalization of (A) PMCA4 (green), (B) CaSRO (red), and (C) Merged image.
Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569]

Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569]

Immunohistochemistry-Paraffin: PMCA4 Antibody (JA9) [NB300-569] - Normal biopsies of deparaffinized Human brain tissue.
Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569]

Immunocytochemistry/ Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569]

Immunocytochemistry/Immunofluorescence: PMCA4 Antibody (JA9) [NB300-569] - Figure 1 illustrates immunolocalization of PMCA4 and CaSRO in human corneal epithelium. (A) Immunolocalization of PMCA4 (green) using NB300-569 on cell membranes of all cells in each layer of hCE. (B) Immunolocalization of CaSRO (red)on cell membranes of hCE. (C) Merged image. Figure 2 illustrates immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.
View 1 more images

Applications for PMCA4 Antibody (JA9)

Application
Recommended Usage

ELISA

1:15000

Flow Cytometry

2 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Frozen

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Western Blot

1:1000
Application Notes
WB: Detects an approx. 133 and 129 kDA protein representing PMCA4a and PMCA4b ATPase respectively. May be useful in Flow.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using NB300-569 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PMCA4

The protein encoded by the PMCA4 gene belongs to the family of P-type primary ion transport ATPases characterized by theformation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ionsfrom eukaryotic cells against very large concentration gradients and play a critical role in intracellular calciumhomeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and thediversity of these enzymes is further increased by alternative splicing of transcripts. The expression of differentisoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting thatthese pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes theplasma membrane calcium ATPase isoform 4. Alternatively spliced transcript variants encoding different isoforms havebeen identified. (provided by RefSeq)

Alternate Names

ATP2B2plasma membrane calcium ATPase, ATPase, Ca++ transporting, plasma membrane 4, DKFZp686G08106, DKFZp686M088, EC 3.6.3, EC 3.6.3.8, Matrix-remodeling-associated protein 1, matrix-remodelling associated 1, MXRA1, Plasma membrane calcium ATPase isoform 4, plasma membrane calcium pump, Plasma membrane calcium pump isoform 4, plasma membrane calcium-transporting ATPase 4, PMCA4b, PMCA4PMCA4x, sarcolemmal calcium pump

Entrez Gene IDs

493 (Human); 29600 (Rat)

Gene Symbol

ATP2B4

UniProt

P23634 (Human)

Additional PMCA4 Products

Product Documents for PMCA4 Antibody (JA9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for PMCA4 Antibody (JA9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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