PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33172
![Protein Array: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172]](https://resources.bio-techne.com/images/products/PMEL17-SILV-Antibody-NKI-beteb-Azide-and-BSA-Free-Protein-Array-NBP2-33172-img0002.jpg "Protein Array: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Equine, Rat (Negative)
Applications
Flow (Intracellular), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # NKI-beteb
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Membranes from a human melanoma metastasis
Reactivity Notes
Does not react with Rat.
Localization
Cytoplasmic
Specificity
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-29407).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free
Protein Array: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172]
Protein Array: PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free [NBP2-33172] - Analysis of Protein Array containing more than 19,000 full-length human proteins using PMEL/PMEL17/SILV Antibody (NKI-beteb). Z-Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.Applications for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunohistochemistry-Paraffin
0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PMEL
Long Name
Premelanosome Protein
Alternate Names
Gp100, ME20, ME20-M, ME20M, PMEL17, SILV
Gene Symbol
PMEL
Additional PMEL Products
Product Documents for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free
Product Specific Notices for PMEL17/SILV Antibody (NKI-beteb) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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