PMEL17/SILV Antibody (PMEL/2037)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07782

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PMEL/2037

Concentration

0.2 mg/ml

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Product Specifications

Immunogen

A recombinant fragment (around aa 376-502) of human SILV protein (exact sequence is proprietary) (Uniprot: P40967)

Localization

Cytoplasmic

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08518)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for PMEL17/SILV Antibody (PMEL/2037)

Western Blot: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782]

Western Blot: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782]

Western Blot: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV Mouse Monoclonal Antibody (PMEL/2037).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782] - Formalin-fixed, paraffin-embedded human Skin stained with PMEL17/SILV Mouse Monoclonal Antibody (PMEL/2037).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/2037) [NBP3-07782] - Formalin-fixed, paraffin-embedded human Melanoma stained with PMEL17/SILV Mouse Monoclonal Antibody (PMEL/2037).
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Applications for PMEL17/SILV Antibody (PMEL/2037)

Application
Recommended Usage

ELISA

2-4 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

2-4 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Gene Symbol

PMEL

UniProt

P40967 (Human)

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (PMEL/2037)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PMEL17/SILV Antibody (PMEL/2037)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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