Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PMEL/783

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all PMEL Antibodies »

Product Specifications

Immunogen

Recombinant human PMEL17/SILV protein (Uniprot: P40967)

Localization

Cytoplasmic

Marker

Melanoma Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from bioreactor concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44522).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free

Western Blot: PMEL17/SILV Antibody (PMEL/783)Azide and BSA Free [NBP2-47779]

Western Blot: PMEL17/SILV Antibody (PMEL/783)Azide and BSA Free [NBP2-47779]

Western Blot: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779] - Western Blot Analysis of COLO-38 cell lysate using PMEL17/SILV Mouse Monoclonal Antibody (PMEL/783).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779] - Human Testis stained with gp100 / Melanosome Monoclonal Antibody (PMEL/783).
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779]

Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free [NBP2-47779] - Human Melanoma stained with gp100 / Melanosome Monoclonal Antibody (PMEL/783).

Applications for PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml

Western Blot

2-4 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: PMEL

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci.

Long Name

Premelanosome Protein

Alternate Names

Gp100, ME20, ME20-M, ME20M, PMEL17, SILV

Gene Symbol

PMEL

Additional PMEL Products

Product Documents for PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PMEL17/SILV Antibody (PMEL/783) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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