PMEL17/SILV Antibody (SPM286) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34755
![Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (SPM286) - Azide and BSA Free [NBP2-34755]](https://resources.bio-techne.com/images/products/PMEL17-SILV-Antibody-SPM286-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34755-img0001.jpg "Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (SPM286) - Azide and BSA Free [NBP2-34755]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Equine, Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # SPM286
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Membranes from a human melanoma metastasis
Reactivity Notes
Does not react with Rat.
Localization
Cytoplasmic
Specificity
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Marker
Melanoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32855).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for PMEL17/SILV Antibody (SPM286) - Azide and BSA Free
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (SPM286) - Azide and BSA Free [NBP2-34755]
Immunohistochemistry-Paraffin: PMEL17/SILV Antibody (SPM286) - Azide and BSA Free [NBP2-34755] - Formalin-paraffin human melanoma tissue stained with gp100 MAb (SPM286).Applications for PMEL17/SILV Antibody (SPM286) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
1-2ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: PMEL
Long Name
Premelanosome Protein
Alternate Names
Gp100, ME20, ME20-M, ME20M, PMEL17, SILV
Gene Symbol
PMEL
Additional PMEL Products
Product Documents for PMEL17/SILV Antibody (SPM286) - Azide and BSA Free
Product Specific Notices for PMEL17/SILV Antibody (SPM286) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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