PML Protein Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-98823

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all PML Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PML Protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PML Protein Antibody

Western Blot: PML Protein Antibody [NBP2-98823]

Western Blot: PML Protein Antibody [NBP2-98823]

Western Blot: PML Protein Antibody [NBP2-98823] - Anti-PML Protein rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa (We are unsure as to the identity of these extra bands).
Immunocytochemistry/ Immunofluorescence: PML Protein Antibody [NBP2-98823]

Immunocytochemistry/ Immunofluorescence: PML Protein Antibody [NBP2-98823]

Immunocytochemistry/Immunofluorescence: PML Protein Antibody [NBP2-98823] - Immunofluorescence staining of PML Protein in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human PML Protein polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.
Immunoprecipitation: PML Protein Antibody [NBP2-98823]

Immunoprecipitation: PML Protein Antibody [NBP2-98823]

Immunoprecipitation: PML Protein Antibody [NBP2-98823] - PML Protein was immunoprecipitated using: Lane A: 0.5 mg A549 Whole Cell Lysate. 4 ul anti-PML Protein rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-PML Protein rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 48-98 kDa. Observed band size: 85 kDa

Applications for PML Protein Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:300-1:10000

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PML

PML Protein is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified.

Long Name

Promyelocytic Leukemia

Alternate Names

MYL, RNF71, TRIM19

Gene Symbol

PML

Additional PML Products

Product Documents for PML Protein Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for PML Protein Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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